LOCUS       40924_45728       14224 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUMLIEP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Okochi-Takada E, Hattori N, Ito A, Niwa T, Wakabayashi M, Yoshida M,
            Ushijima T.
  TITLE     Establishment of a high-throughput detection system for DNA 
            demethylating agents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14224)
  AUTHORS   Hattori N, Ushijima T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Contact:Naoko Hattori National Cancer Center
            Research Institute, Division of Epigenomics; 5-1-1 Tsukiji, Chuo-ku,
            Tokyo, Tokyo 104-0045, Japan
REFERENCE   3  (bases 1 to 14224)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14224)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2016) Contact:Naoko Hattori National Cancer Center Research 
            Institute, Division of Epigenomics; 5-1-1 Tsukiji, Chuo-ku, Tokyo, 
            Tokyo 104-0045, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    698..6262
                     /gene="UCHL1"
                     /label=UCHL1 5'region
                     /note="UCHL1 5'region"
     gene            698..6262
                     /gene="UCHL1"
                     /label=UCHL1
     misc_feature    6225
                     /gene="UCHL1"
                     /label=UCHL1 TSS
                     /note="UCHL1 TSS"
     CDS             6316..6972
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
     misc_feature    6988..7574
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7575..8291
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    8417..8538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8562..8578)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8603..9410
                     /gene="UCHL1"
                     /label=contains UCHL1 intron 4
                     /note="contains UCHL1 intron 4"
     gene            8603..9410
                     /gene="UCHL1"
                     /label=UCHL1
     promoter        9420..9749
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             9842..10438
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    10671..10805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    10805..12017
                     /gene="UCHL1"
                     /note="contains UCHL1 intron 4, exon 5, intron 5, exon 6,
                     and intron 6"
     gene            10805..12017
                     /gene="UCHL1"
                     /label=UCHL1
     promoter        complement(12036..12054)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12075..12091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12099..12115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12123..12153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12168..12189)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12477..13065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13239..14096)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(14097..14201)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatcta ggcggcacag caagaccctg
      721 tttcaaaaca aaaacaagac cagtcgccgt ggctcacatc tgtaatccca gcactttggg
      781 aggccgagga gggtggatca cctgaggtca ggagttcgag accagcctga ccaacatggt
      841 gaaaccccat ctctactaaa aataaaaatt agtcgggcgt ggtggcacat gcctataatc
      901 ccagctactc gggaggcttg aggcaggaga atcgcttgaa cccgggaggc ggaggtggca
      961 gtgagccaag actgcaccac tgcactccag cctgggcaac aagagcgaaa ccctgtttca
     1021 aaaaacaaaa acagaaacaa aaaacaaaca aaccacaaaa agcctctgac aaatagagct
     1081 gggaaaagcc aggaagagaa tgattctcat gcataaatgc ctgataacaa aaattatcac
     1141 aaaagactgc aaaacccaca actttgtaca aaggccatca cgatcttaca taaaaaatcc
     1201 ttctgcaagg acttgtgcct agaaactgct tgtccagcct cagactggtg tcacccttgt
     1261 tattgatcct tgtagccaag gataattatt tcaaaacaat tatgtaatat tcctcatttg
     1321 tccttaaaaa acctttgtct tcttgtatct tcctgaatat acacacagtt tactatggca
     1381 tgtgtatttc cattgcaatg ccctattcct gaataaatat cactttcttt tagagaacat
     1441 ttctctgtta tttatgttga cacatggaca accctagaac caccaacctc cagtcattga
     1501 gttaagagag ggaattcatg tcctaagctt tcagtcagtt ttagttgggt attctgccct
     1561 tggtagacat agtataatac agcggtcccc agcctttttg gccccaggga ccagtttcat
     1621 ggaagacaat ttttccacgg actgggggtg ggaatggttt cgggatgaaa ctgttccacc
     1681 tcagatcatc agtcattaga ttctcataag aagcacacag cctagatccc tcgcatgtgc
     1741 agttcataat agggtttatg ctcctatgag aatctaatac tgctgctgat ctgacaggag
     1801 gcggagctca ggcagtaatg tttgctcacc cgccgctcac ctcttgctgt gccacccagt
     1861 tcctaacagg ccacagacct gtaccagtcc tcagcctggg acttgaggac tcctggtata
     1921 atatagtaca gtggtcaaga gtgcagactg tctgtggtca aatttcagtt ctcatgactt
     1981 aaataaccat aggcaaagaa cttaaccttt ctgatcttca tttctaaatg agaataataa
     2041 tagtaaccta cctaataggt tgttatgttg attaaaagag ttaatacaca gaaaacacaa
     2101 agaagtagtg cctggcatcc gtaagcaatc agtaagtgtt agctatgatt atgctgctaa
     2161 aagcatgctc accattacat gtgaagaatt tacataaatg ttgaaccact ctaattatta
     2221 agtgctttct tatataaact aatattttca tctgacttct acctgtctgt cctgtattct
     2281 taaataatca taaagattat tctcgccctc tttgtatttt tgagcatttg gtatcatgta
     2341 actcccctcc ccatccccca aacacacaca caaacccttt ctcttctcca catcatatac
     2401 tgtcttctca atttcttcaa ccacctttgt tcattatttt ggcgctcagt ctctgaaaac
     2461 tctctaatat gtcaatgtct cttttgaagt gtggtagttc gaattttcag ttgaaaagta
     2521 tttattgagt gttcattata tgccaggctg ggcatcataa tccagttata gtctggtcaa
     2581 ttctgtgagt ggattagatc ttgaatatta tattaatcta gccaaaggtt agcaaatacc
     2641 tggttatcct taccacttcc ctttcccata ctcgtggtag acatcactaa tcaatcacag
     2701 catttttccc cctactggac tcaggactct ccttcaggaa cctatgatta aaatgaaatt
     2761 gaatctctca ctcctggtat aaataaagat tgcactagct ttatagtcaa attatgacac
     2821 ctgaaatatt tgaatctttt tctcaggtac tgttcacaaa gccagatcta attcattaat
     2881 gcatctgtaa atgatttttt ttaaaaaatg tttttccttc tgatctctgt gtatttattt
     2941 ttaaaaggtt acaatcaaac tattgtgttg ttttcacttg atattttcca aattatttca
     3001 tacttttgca atcatagcat caagaaaaaa gttaatataa agattaactg tatggcaata
     3061 attgacaact gttactataa tatagagctt tacacatatt cctgttacat ttttaatagc
     3121 tttattgtga tacctaaacc cactttttag ttttttagta tattcacagg gttatgtaat
     3181 cgtcacctaa atccctgtta aatttcatca ttttatttta aacttatttt ttaatcccaa
     3241 aattctgcca tttattatgt atttatttat tttaagagat ggggtctcac tctgttgccc
     3301 aggttggaat gcagtcatgc catcacagct cattgtagcc tcgaactcct gggctcaagc
     3361 aatcctccca ctttggcctc tcaagtagct gggactgcag gtacacacca ccacacctgg
     3421 ctaagaattc tgccatttag actgcttgtt cttgcttgta tgcacgacct ctctttttct
     3481 ctgctgtaat aaagtggcac cagacaaggt taaaaccatt gtcaggtggc tacaggttga
     3541 ggtagctcca tccagtacca cttttttttt taaggtagaa tcaattaaat aagctataaa
     3601 accacctcaa ctgtggtatc aaccaatgcc aaccctggca gcataagaaa tacaatgaca
     3661 gtgttctacc tgtccatcca gttaataacc cccaaagaaa agaagataag ttctggctgg
     3721 cataacttgt tctaaatgca tccatcttat tgtttctaaa agtaaaaaat tatgagtcct
     3781 aacatttggc taatgactga tataaactct tctggcctgc agtttcagca gtatattccc
     3841 aatcaccccc tacttcttct tcttcttctt tttttttttt ttaaaaaaga ttaggcttag
     3901 gtttgttcat atccagtctc tggcatctct ttgtaagcag ggagttgttt cattcactgc
     3961 tctgatgctt cctggcaaac agtaggccat atatgtatat gaatgtgaat gaattaatgc
     4021 cttcaacaaa tatttctgac agtgatttca tgactgcttt tattagttat tttggcaatc
     4081 acttataact gctaacgtct gcttgccgat cagcccttag cttaaatgtc acttccttca
     4141 ggacgccttc ctgatcccct ccactaggtt aggtgctcct gatctctgct ccctttcata
     4201 caactcttat gactgataaa aacagcacga gggggccagg cgcggtggct cacgcctgta
     4261 atcccagcac tttgggaggc cgagacgggc ggatcacgag gtcagcagat gagaccatcc
     4321 tggctaacac ggtgaaaccc cgtctctact aaaaatacaa aaaaaattag ccgggtgtgg
     4381 tggcgggcgc ctgtagtccc agctactccg gaggctgagg caggagaatg gcgtgaaccg
     4441 gggaggcgga gcttgcagtg agccgagatc gcgccactgc actccagctt gggcgacaga
     4501 gcgagactcc gtctcaaaac aaaacaaaca aaacaaacaa acaaaaaaac ggcatgaggg
     4561 aaatttccat gtttattgtg ttcatctttg tatgcctaat tccaaacttt ccaagctcgg
     4621 cacccagcac acagtaggtt cttaatattt gttgagtgaa tgaataaatg aagtaaattg
     4681 gaaatgtaat ttgtctttgt ctaaaggcaa gaactcattc aatataacct agtcacttgt
     4741 cttgaccttc acctcttttt ttctttaaca accttattga gggaaaattt acataccata
     4801 agattcaccc attaagctcc tcttactaat ttttttctct ctcatttttg tgtgtgaagg
     4861 tcattttcta atggcaaaaa tggaaagaaa atagaagtag aatgttgtgg aatcttagat
     4921 aaagaatggg ctcttggagc ccagtttagc agggtttact tctcagtttt acttttcaat
     4981 tgtgaccgta agcaaattaa cttctctagg cctgggattc tgatctgtaa aattgcacta
     5041 atatgagtct cttcacggct cctctaagga ttaaatgaga gacacatgca aaggatcccc
     5101 aaaaacaata actcaaaaaa tgttgattcc ctcccttccc tctgtcatct gttaacctca
     5161 acttcctaaa tagaaggtct attcttttac catcatcatt attctcttcg gtgcctattt
     5221 ttaaaaaata ctcaaccttc ttgcttcctt cgctacctaa gtatttctgc aagcccactt
     5281 tgttctgcag cttagctttc ctggcacaat tcttatagat tttggtccct tttaaaattc
     5341 attcttcagc aaatgctttc tctctccatc ttttgactag agatcattag agatcacctg
     5401 agatcattag agaacagtgg tttccttggt tgccattccc tttcttcttc attgggagta
     5461 ttctgcggtg aactcagaca ttttattttt caaagcttcc cattctttta aaaatgcttt
     5521 tccttttaca gcctctcgct caaaatcata cccatctttt ccctggatct gttttctcaa
     5581 gtctccaatc gcctgccttc tttgtgtctt gtattaccct cacatccccc agctttctac
     5641 tgctctccca ggaccaacca tttcttccgc gggagtcaca ttacatcagc attcctaatg
     5701 cagtatctgt tatctaccag attctgtttt attctaggta gtcacttaaa aacgaacctc
     5761 ggtactggtc tgacttaaca tggaggagga attgtctaag gttaaacgca aactgctgag
     5821 agatttgggg cggggggcac acatttacat tcattcgtat taaatatata cctgttgaat
     5881 ttgtgctttt tctcaaatgc ttcagagact cgagctttag agtaattggg atggtgaaag
     5941 gatgggtttc cagaaacttc gcccaaaatt aaagactcca tcaaaaggac tgctccatac
     6001 actcaaggaa cacccaccaa caaatcccgt ctccacaacc accagattat ctcaccggcg
     6061 agtgagactg caaggtttgg gggcccggcc gtaccactcc gcgctgcgca cggggggttc
     6121 gtacccatct ggccgcgacc gtccgtttcc ccctcgcttg gttctgcccc tgctccccct
     6181 gcacaggcct cacagtgcgt ctggccggcg ctttatagct gcagcctggg cggctccgct
     6241 agctgttttt cgtcttccct agatcgaatt ctgcagtcga cggtaccgcg ggcccgggat
     6301 ccaccggtcg ccaccatgga catcaaggtg gtgttcaccc tggtgttcag cgccctggtg
     6361 caggccaaga gcaccgagtt cgaccccaac atcgacatcg tgggcctgga aggcaagttc
     6421 ggcatcacca acctggaaac cgacctgttc accatctggg agaccatgga agtgatgatc
     6481 aaggccgaca tcgccgacac cgaccgggcc agcaacttcg tggccaccga gaccgacgcc
     6541 aaccggggca agatgcccgg caagaagctg cccctggccg tcatcatgga aatggaagcc
     6601 aacgccttca aggccggctg cacccggggc tgcctgatct gcctgagcaa gatcaagtgc
     6661 accgccaaga tgaaggtgta catccccggc aggtgccacg actacggcgg cgacaagaaa
     6721 accggccagg ccggcatcgt gggcgccatc gtggacatcc ccgagatcag cggcttcaaa
     6781 gaaatggccc ccatggaaca gttcatcgcc caggtggaca gatgcgccag ctgcaccacc
     6841 ggctgcctga agggcctggc caacgtgaag tgcagcgagc tgctgaagaa gtggctgccc
     6901 gaccgctgcg ccagcttcgc cgacaagatc cagaaagagg tgcacaacat caagggcatg
     6961 gccggcgaca ggtgagcggc cgatccgccc ctctccctcc ccccccccta acgttactgg
     7021 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     7081 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7141 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     7201 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     7261 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     7321 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     7381 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     7441 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     7501 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     7561 atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     7621 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     7681 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     7741 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     7801 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     7861 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7921 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7981 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     8041 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     8101 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     8161 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     8221 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     8281 agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     8341 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     8401 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8461 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8521 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tgatccacta gttctagagc
     8581 ggccgccacc gcggtggagc tcagccatcc taagcttgaa cttgaaacat ggagttcaga
     8641 aacagctgtt ttcccgaggt cagagatgct gtaccttttg aaaccatttt gtaatgatgg
     8701 tgtcagacac ttaatctgac tcacagtcct cctcagaaag gattcctagc acttccaaat
     8761 tttgaaatgg tcccatttca caaattttag agctgaaagt tcatattgag gtcttttgaa
     8821 atatacattg ttattgctat aggtttgtaa ttagtgtctc ttaagcattt tgttaatgct
     8881 cagtgtggat aagcctatgt cagtggttag ccagtcagat gtctctatgc tggactattt
     8941 tgtttcttta catttctatt caagatcttt atttttctat ctgaataacc gaattactaa
     9001 agttgaattt tctaccccat aatcttggtt tgggaagcat ttttattaga acatgattat
     9061 agcgtcatgt atataataga tgttaactgt acattcttag atgctgtaga tacagcaggt
     9121 aaacaaggca gccccggctt ttatggtgct agaaatctca aatatgtttt acaaattaag
     9181 aattgctgtg aaaaagtgac aggagatttg agagatgagt gaggtgggag ggaaaaaggg
     9241 gtccagggta gaagggaaca gaggcaagaa agagcttggc atattgggag agcggagaga
     9301 cctctctggc ctgaaagcag tgaatagaga ggggttagaa aaagaggcta ggggaaaagg
     9361 tacagctcat ccacaaccct ggaggcagta ttaaagattc aggttgctca ctgtggaatg
     9421 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9481 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9541 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9601 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9661 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     9721 gcttttttgg aggcctaggc ttttgcaaaa agcttgcatg cctgcaggtc ggccgccacg
     9781 accggtgccg ccaccatccc ctgacccacg cccctgaccc ctcacaagga gacgaccttc
     9841 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     9901 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     9961 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
    10021 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
    10081 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
    10141 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
    10201 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
    10261 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
    10321 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
    10381 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
    10441 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atggctccga
    10501 ccgaagccga cccgggcggc cccgccgacc ccgcacccgc ccccgaggcc caccgactct
    10561 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    10621 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    10681 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10741 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10801 ggatctcagc atgttcagca aaggcttaag tcaacaataa atatgtaccc acttgtatta
    10861 ttttacctat actaacacat ccattttttt tttaagagga tggatcagtt ctgaaacagt
    10921 ttctttctga aacagagaaa atgtcccctg aagacagagc aaaatgcttt gaaaagaatg
    10981 aggtaagaga acttacagag catggccttt aaataactct agagattttt gtgctaatta
    11041 ttttcttttt tccgcaggcc atacaggcag cccatgatgc cgtggcacag gaaggccaat
    11101 gtcgggtaaa tgcaaataca aatcggagcc aggctgcctg ggtgccatct gtgtttctac
    11161 tgaaattgtg caggaatctc ttactggaac acagcaaatg ttatccaccc aaggccaaat
    11221 gacaccagaa agttctaccc aaataatgct ttgactagac ctgttcataa aaaaagtttt
    11281 cttcagaaat tgtattggtc attgaagtct tgagtaaatt caaattgaca gtattttgtt
    11341 tagatccaaa tgagtcttat aggtcaggca tagtggctca tgcctgtaat cccaacaatt
    11401 tgggaggccg aggagggtga atcacttgag cccaggagtt tgagaccagc ttggccaaca
    11461 tagtgaaacc ccgtccctac aaaaaataca gagtagcgag gtctgtagtc ccagccactt
    11521 gggaggctga ggtgggagaa ttgcttgagc cctagaggca gaggttgcag tgagctgaga
    11581 ccatgtcgct gcactccggt ctgggtgacc ctgtctcaat aacagaaaca aatgagtctt
    11641 atagagtgta cacctctcct agtaagtgag catttcaaat aatcttcaca ggctgttggg
    11701 gagaatgaaa tgcccaacga aggacaagtt ctagaaggct tgaatatacc ataatctgat
    11761 gagaacctct gagtcagctg ctaaatttaa gtcagatctc agtgtccttt ttaaaaaaaa
    11821 aaaaaagttg acaaggtcag cctccatttc ccaggctgga atgcagtggt gtgatcttgg
    11881 ctcactgcag caattctctc atctcagcct cccgagtagc tgggattaca agtacacacc
    11941 accacaccta ctaattttta tattttttgt agagatgggg tttcaccatg ttgttcaggc
    12001 tggtcttgaa ctcttgtgag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
    12061 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    12121 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    12181 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    12241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    12301 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    12361 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    12421 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    12481 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    12541 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12601 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12661 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12721 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12781 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    12841 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    12901 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    12961 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    13021 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    13081 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    13141 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    13201 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    13261 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    13321 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    13381 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    13441 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    13501 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    13561 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    13621 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    13681 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    13741 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    13801 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    13861 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    13921 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    13981 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    14041 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    14101 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    14161 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    14221 ccac
//
